Global Experts Meeting on Infectious Diseases
National Research Center
Title: Medical and microbial applications of controlled shape of silver nanoparticles prepared by ionizing radiation
Biography: Mohamed Nasr Fathi Shaheen
New strategies to develop the efficacy of drugs are required to treat the emerging infectious diseases and microbial resistance. A new effective strategy is to design drugs combined with metal nanoparticles to overcome infections of multidrug-resistant microbes. In this study, three different forms of silver nanoparticles (S1-S3) were prepared. The three forms were investigated against coxackieviruses and 5 fungal strains (against Aspergillus fumigatus, Candida albicans, Geotricum candidum, Aspergillus niger, Trichophyton mentagrophytes). For antibacterial and anticancer evaluation, S2 was used. All forms showed antiviral activities against virus replication with TI ranged from 0.4 to 30 and reduction in virus titers ranged from 0 to 4 log10 TCID50, where S3 showed the maximum antiviral effect followed by S1 and S2. On the other hand, Ag-NPs S2 exhibited antifungal activities against 5 fungus, mostly against Trichophyton mentagrophytes. No antifungal activities were observed from S1 and S3 forms. Ag-NP S2 showed antibacterial effects against gram negative Escherichia coli, Acinetobacter baumannii, Klebsiella pneumonia, Salmonella typhi, and Enterobacter cloacae) and positive bacteria (Bacillus subtilis, Staphylococcus aureus, Streptococcus mutans). In contrast, no activity was observed from Ag-NP S2 against some negative bacteria (Proteus mirabilis and Serratia plymuthica) and positive bacteria (Micrococcus boride, Staphylococcus epidermidis, and MRSA). Furthermore Ag-NPs displayed a significant cytotoxic potential in cancerous Hep-G2 and A549 cell lines. These findings indicate that Ag-NPs can be utilized as antimicrobial and anticancer agent.