Human astroviruses have been increasingly identified as importantrnagents of diarrheal disease in children. Outbreaks of diarrhea due tornastrovirus have frequently been reported and astroviruses have alsornbeen associated with nosocomial infections in hospitals. The aim ofrnthis study is detection of astrovirus in hospitalized children withrngastroenteritis.rnIn this 7 years descriptive study 2,490 gastroenteritis samples werernconducted to determine the prevalence and age distribution ofrnhuman astrovirus infection as well as the seasonality pattern in 5rndifferent cities of Iran using reverse Transcriptase-polymerase chainrnreaction (RT-PCR).rnAstrovirus was detected in 40 of 2490 specimens tested by RT-PCR,rnand astrovirus infection was confirmed by Southern hybridization.rnDetection rates were higher in winter, although astrovirus infectionsrnalso occurred in summer months. The overall incidence of astrovirusrnwas found to be 1.6%. The mean age of infected children was 14.7rnmonths, the median age was 15 months. Majority of the infectedrnchildren were less than 2 years of age making up 36 (90%) infectedrnchildren, only 4 cases of infected children were more than 2 years ofrnage (10%). The difference between the two age groups wasrnstatistically significant (P < 0.02).rnOur findings provide Evidence that astrovirus can be a leadingrncause of viral gastroenteritis infections and highlight the need tornimplement astrovirus detection assays in association with rapidrnrotavirus and adenovirus detection enzyme immunoassays (EIAs)rnfor the clinical diagnosis and nosocomial prevention of viralrnGastroenteritis infections in pediatric departments.
I am Dr. Saeid Maham,the mnagmer of Pediatric Infections Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. is My inteest is resaerch in medicaland diagnostic methods filed. I interest to participate in scientific congress toshare my knowledge and data with other researchers.
Bloodstream infectious diseases are prevalent in children and adults. Rapid and accurate diagnosis of bloodstream infections primary based on conventional culture results, which saves time and prevents empiric treatment. This study aims to BACTEC and Conventional Culture Systems Comparison to Isolat of Microorganisms from Blood and Other Sterile Body Fluids, on blood specimens collected from three training hospitals in Tehran, Iran. BACTEC 9120 and conventional methods were used for isolation of microorganism from specimens including blood and other body fluid samples collected from patients hospitalized in the selected hospitals during the study period, from April to June 2009. Time for positive and negative results and hospital charge were estimated for the two culture methods. In total 747 specimens were possessed by BACTEC 9120 systems and 787 by conventional method. Patients aged between 3 days and 8 years old, (mean 11.4±21.9 years); 52% of patients were male and 48% female; Out of 747 specimens were possessed by BACTEC 9120 system, 26% (196/747) and from 787 specimens cultured by conventional method 5% (49/787) were positive (p<0.05). Hospital stay was 13.8 ± 12.9 days in BACTEC 9120 and 17.9± 14.9 days in Conventional method,(p<0.05) respectively. Time for positivity by BACTEC system was 3.8 ± 1.1 Days and 5.9 ± 2.5 days in conventional method.(p<0.05), treatment response showed 1.8 day earlier result in patients that their specimens were processed by BACTEC 9120 system . Death rate in BACTEC 9120 method was 6% and 11% in Conventional method. (p>0.05).In conclusion implementation of BACTEC system for microbiologic detection of pathogens decreases the admission time and early diagnosis and treatment results are cost effective for patient management and prevention of antibiotic resistance.